SanPrep Column Plasmid Mini-Preps kit |
Sangon (Shanghai, China) |
DNA purification kit |
TaKaRa (Dalian, China) |
Restriction enzymes |
TaKaRa (Dalian, China) |
ClonExpress MultiS One Step Cloning kit |
Vazyme (Nanjing, China) |
PrimeScriptRT reagent kit |
TaKaRa (Dalian, China) |
RevertAid First Strand cDNA synthesis kit |
Thermo Scientific (USA) |
PrimeSTAR HS DNA polymerase |
TaKaRa (Otsu, Japan) |
Glucosamine (GlcN) |
Sigma-Aldrich (St. Louis, MO, USA) |
ortho-Phthalaldehyde (OPA) |
Sigma-Aldrich (St. Louis, MO, USA) |
Dithiothreitol (DTT) |
Sigma-Aldrich (St. Louis, MO, USA) |
Ampicillin (Amp) |
Amresco (Solon, OH, USA) |
Isopropyl-β-d-1-thiogalactopyranoside (IPTG) |
Merck (Darmstadt, Germany) |
Other chemicals |
Sangon (Shanghai, China) |