Table 1.
Summary of the transcriptome bioinformatics pipeline.
| Sample | Raw | Trimmed |
|---|---|---|
| C2 t0 | 78,426,948 | 99.59% |
| C3 t0 | 44,346,854 | 98.03% |
| C4 t0 | 100,814,198 | 99.59% |
| I1 t0 | 17,696,894 | 98.08% |
| I2 t0 | 93,114,098 | 99.60% |
| I10 t0 | 96,780,602 | 99.64% |
| C2 24h | 95,296,484 | 99.49% |
| C3 24h | 51,708,988 | 97.62% |
| C4 24h | 92,661,282 | 99.65% |
| I1 24h | 52,102,302 | 98.77% |
| I2 24h | 90,965,875 | 99.52% |
| I10 24h | 99,262,970 | 99.55% |
| C2 24h2 | 93,665,372 | 99.96% |
| C3 24h2 | 47,632,114 | 99.09% |
| C4 24h2 | 83,629,996 | 99.62% |
| I1 24h2 | 25,833,124 | 98.25% |
| I2 24h2 | 90,636,926 | 99.60% |
| I10 24h2 | 101,411,946 | 99.64% |
| C2 7d2 | 78,528,396 | 99.55% |
| C3 7d2 | 46,511,774 | 99.36% |
| C4 7d2 | 95,180,866 | 99.69% |
| I1 7d2 | 13,593,322 | 97.43% |
| I2 7d2 | 90,614,296 | 99.31% |
| I10 7d2 | 98,236,250 | 99.57% |
| Assembly statistics | ||
| Contigs | 260,664 | |
| Range contig length | 200–15,624 | |
| Average contig length | 512 | |
| N50 | 576 | |
| Complete metazoan BUSCOs | 740/978 (75.69%) | |
| Fragmented metazoan BUSCOs | 218/978 (22.29%) | |
| Missing metazoan BUSCOs | 20/978 (2.04%) | |
| Blast | ||
| Contigs with hit in Uniprot/SwissProt | 19.93% | |
| Contigs with hit in mollusks database | 42.64% | |
| GO analysis | ||
| Annotated contigs | 23.35% | |
| KEGG analysis | ||
| Pathway assigned contigs | 7.27% | |