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. 2019 Jul 30;3(3):036102. doi: 10.1063/1.5094735

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© Author(s).

2473-2877/2019/3(3)/036102/12

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FIG. 4. — Effect of VEGF flux on 3D network formation. (a) The representative 3D projected images of endothelial networks on day 5. Actin filaments were stained in green. (b) An enlarged image shown in the rectangle in (a). The arrowheads indicate the cells detached from network tips, while the arrows indicate separation of endothelial networks. (c)–(e) Quantitative analysis of the network length and density and area of degraded gel. Data = mean ± SD; n = 15–33 (c), n = 8–10 (d) and (e). *P < 0.05 vs the other conditions. **P < 0.05 vs 25 mmH₂O with 20 ng/ml VEGF. †P < 0.05 vs the other conditions except for 25 mmH₂O with 20 ng/ml VEGF. ††P < 0.05 vs the other conditions except for 25 mmH₂O with 20 ng/ml VEGF. Scale bars, 300 μm (a) and 150 μm (b).