FIG. 6.

(a) The average percentage of highly metastatic PC3 prostate cells that formed blebs $\pm$ SD (left, 1000 Pa: $\mathrm{n}=167$, 2000 Pa: $\mathrm{n}=155$, 3000 Pa: $\mathrm{n}=213$, 4000 Pa: $\mathrm{n}=219$, no statistical significance, one-way ANOVA), average cell fluidity $\pm$ SD (center, 1000 Pa: $\mathrm{n}=35$, 2000 Pa: $\mathrm{n}=29$, *$\mathrm{p}=0.0065$), and average cell stiffness $\pm$ SD (right, **$\mathrm{p}\ll 1\times {10}^{-6}$). (b) The average percentage of moderately metastatic DU145 prostate cells that formed blebs $\pm$ SD (left, 1000 Pa: $\mathrm{n}=185$, 2000 Pa: $\mathrm{n}=215$, 3000 Pa: $\mathrm{n}=221$, 4000 Pa: $\mathrm{n}=177$, no statistical significance, one-way ANOVA), average fluidity $\pm$ SD (center, 1000 Pa: $\mathrm{n}=26$, 2000 Pa: $\mathrm{n}=35$, *$\mathrm{p}=0.0019$), and average stiffness $\pm$ SD (right, **$\mathrm{p}=0.0075$). (c) The average percentage of normal RWPE-1 prostate cells that formed blebs $\pm$ SD (left, 1000 Pa: $\mathrm{n}=241$, 2000 Pa: $\mathrm{n}=245$, 3000 Pa: $\mathrm{n}=255$, 4000 Pa: $\mathrm{n}=303$, *$\mathrm{p}=0.0032$, **$\mathrm{p}=0.0011$, ***$\mathrm{p}=0.0002$, one-way ANOVA followed by Tukey’s post hoc test), average fluidity $\pm$ SD (center, 1000 Pa: $\mathrm{n}=30$, 2000 Pa: $\mathrm{n}=16$, *$\mathrm{p}=0.047$), and average stiffness $\pm$ SD (right, **$\mathrm{p}=0.025$). Averages were taken over 3 or more independent experiments. Each pressure drop used a new set of cells. Statistical comparisons between two groups used the two-sample Kolmogorov–Smirnov test.