TABLE III.
Summary of the main topics covered in the reviews on CTCs.
| Reference | Key topics discussed in the corresponding review paper | Other topics/Remarks | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Biological/properties of CTCs | Summary of CTC capture methods | Comparison table of CTC capture devices: I/WO = including commercial | 2 isolation methods | CTC characterization methods | Clinical applications/relevance of CTCs | CTC culture | EMT | Other tumor cell types (CTCls, CSCs, etc.) | Nanodevices and/or nanomaterials | |||||
| Label-free | Affinity-based | Functional based | In vivo | |||||||||||
| 4 | Y | I | Y | Y | Y | Y | ||||||||
| 18 | I | Y | Other circulating tumor materials (ctNAs, exosomes, etc.) | |||||||||||
| 90 | Y | Y | Y | Ia | Y | Y | ||||||||
| 89 | Y | Y | Y | Y | DTCs properties & isolation | |||||||||
| 93 | Y | Y | I | Y | Y | |||||||||
| 56 | Y | Y | I | Y | Y | |||||||||
| 65 | Y | Yb | I | Yb | Y | Y | Other circulating tumor materials (ctNAs, exosomes, etc.) | |||||||
| 111 | Y | I | Y | Roadmap for clinical validation and qualification of CTC assays | ||||||||||
| 66 | Y | Yb | Y | Y | High flow rates = high throughputs | |||||||||
| 67 | Yc | Yc | ||||||||||||
| 84 | Y | Y | WO | |||||||||||
| 99 | Y | Y | CS | |||||||||||
| 102 | Y | Y | WO | Y | Y | |||||||||
| 81 | Y | Yb | I | Y | Y | Y | Cell release for post-separ. Analysis | |||||||
| 82 | Y | Y | I | Y | ||||||||||
| 83 | Y | Y | WO DEP | Y | Y | Positive vs negative enrichment | ||||||||
| 85 | Y | Y | Y | I | Y | Y | Cell-release for post-separation and analysis | |||||||
| 86 | Y | Y | Y | Y | Y | Y | NMR material for CTC detection | |||||||
| 87 | Y | Y | I | Y | Y | Y | ||||||||
| 92 | Y | Y | Y | Y | ||||||||||
| 91 | Y | Y | Y | Y | Y | |||||||||
| 88 | Y | Y | Y | Y | Y | Y | ||||||||
| 94 | Y | Y | Y | I | Y | |||||||||
| 95 | Y | Yb | Y | Y | Y | Y | Y | |||||||
| 96 | Yd | Y | Y | Positive vs negative enrichment | ||||||||||
| 97 | Yd | |||||||||||||
| 98 | Y | Y | CS /CT by electric & magnetic methods Microsystems best solution for CTC isolation requirements | |||||||||||
| 100 | Y | WO | Y | EIS - possible characterization tool | ||||||||||
| 77, 103, 104 | Y DEP | DEP combine the high purity isolation with conservation of cell membrane DEP - potential of identifying cells with different phenotypes | ||||||||||||
| 105 | Y | Y | I | Y | Y | Y | ||||||||
| 118 | Y | Y | Y | Y | ||||||||||
| 119 | Y | Yb | WO | Y | Y | Y | ||||||||
| 107 | Y | Yb | I | Y | ||||||||||
| 110 | I | |||||||||||||
| 120 | Ye | Ye | ||||||||||||
a
Only immunological.
b
The usage of aptamers used for that specific purpose (affinity-based capture OR detection/characterization).
c
Fundamental mechanisms of cell adhesion and transport.
d
Only immunomagnetic-based.
e
Only using polymers for CTCs' isolation; CS/CT = Focus on “general” cell sorting (CS) OR cell trapping (CT) methods rather than specifically for CTC capture.