TABLE IV.
Summary of previously reported microfluidics devices for isolation and characterization of cfNA.
| Disease | Biomarker | Detection technique | Reference |
|---|---|---|---|
| Lung cancer | Change in DNA content | Microfluidic single molecule spectroscopy | 247 |
| Several cancer types | Single-nucleotide polymorphism—SNP | Electrokinetically driven microfluidic control | 250 |
| Oral cancer | Single-nucleotide polymorphism—SNP | Magnetic beads-based “sandwich” hybridization | 251 |
| Breast cancer | TSG mutation | Integrated waveguide for microarray | 253 |
| Lung cancer and melanoma | Oncogene mutation | Electrochemical clamp assay | 254 |
| Several cancer types | Oncogene mutation | Droplet-based digital PCR Single Color Droplet Digital PCR | 255 |
| Ovarian cancer | DNA methylation | Digestion-based PCR assay | 270 |
| Colon cancer | DNA methylation | Microfluidic-based surface plasmon resonance (SPR) detection | 280 |
| Pancreatic cancer | miRNA in exosome | SAW lysis and ion-exchange nanomembrane detection | 276 |
| Glioblastoma | miRNA in exosome | Immunoaffinity, antibody-binding | 281 |
| Melanoma | miRNA in exosome | Electrophoresis-driven microfluidic filtration | 282 |
| Glioblastoma | miRNA | Differential interference contrast (DIC) imaging technique | 277 |
| Breast cancer | miRNA | Photonic crystals fabricated from silicon, and resonance coupling laser scanning | 278 |