Figure 2. Denitrifying activities of the Ref300N-23C biofilm cultures impacted by different physico-chemical parameters.

The Ref300N-23C biofilm cultures were exposed for few hours or few days in modified ASW medium as described in Table 1. Denitrification rates are expressed relative to the denitrification rates of the original conditions of the Ref300N-23C biofilm cultures (set to one) identified by *. Significance between the denitrification rates were determined by one-way ANOVA. Rates with the same letter are not significantly different. X: no activities recorded. Average of triplicates cultures except for the 400N-NO₂, 200N-NO₂ and 200N-NO₃/200N-NO₂ conditions with one replicate. N: Nitrogen NO${}_3^{-}$. M: methanol.