Table 2. Culture conditions used for the cultivation of the original biofilm.

Name	Medium	NO3−/NO2− mg-N/L (mM)	Methanol % (v/v)	NaCl % (w/v)	Temp °C
Ref300N-23Ca	ASW	300 (21.4)	0.15	2.75	23
Oxicb	ASW	300 (21.4)	0.15	2.75	23
300N-30C	ASW	300 (21.4)	0.15	2.75	30
900N-23C	ASW	900 (64.3)	0.45	2.75	23
900N-30C	ASW	900 (64.3)	0.45	2.75	30
0%NaCl	ASW	300 (21.4)	0.15	0	23
0.5%NaCl	ASW	300 (21.4)	0.15	0.5	23
1%NaCl	ASW	300 (21.4)	0.15	1.0	23
5%NaCl	ASW	300 (21.4)	0.15	5.0	23
8%NaCl	ASW	300 (21.4)	0.15	8.0	23
2.75–5%NaClc	ASW	300 (21.4)	0.15	2.75/5	23
IO	IO	300 (21.4)	0.15	3.0d	23
200-200N	ASW	200 NO3−/200 NO2− (28.6)	0.15	2.75	23

Notes.

The original biofilm was cultivated in triplicates in these conditions at pH 8.0. The carriers were transferred five times in fresh medium (eight times for the 200-200N biofilm cultures). In gray are changed parameters from the Ref300N-23C biofilm cultures. IO: Instant Ocean medium.

^aReference biofilm cultures.

^bCultures were performed under oxic conditions.

^cThe Ref300N-23C biofilm cultures were further transferred three times in ASW composed of 5% NaCl.

^dBased on the amount of Na⁺ and Cl^- in Table S1. The exact amount of NaCl added in the IO medium is not known. For comparison, the amount of Na⁺ and Cl^- in the ASW medium is 3.2%.