Table 1. Biofilm culture conditions.

Name	Medium	NO3−	Methanol	NaCl	Temp	Specifica denitrification rates
		mM (mg-NO3-N/L)c	% (v/v)c	% (w/v)	°C	mM-NOx h−1 mg-protein−1
Ref300N-23Cb	ASW	21.4 (300)	0.15	2.75	23	0.0530
300N-30C	ASW	21.4 (300)	0.15	2.75	30	0.0946
900N-23C	ASW	64.3 (900)	0.45	2.75	23	0.0637
900N-30C	ASW	64.3 (900)	0.45	2.75	30	0.0979
0%NaCl	ASW	21.4 (300)	0.15	0	23	0.0911
0.5%NaCl	ASW	21.4 (300)	0.15	0.5	23	0.0712
1%NaCl	ASW	21.4 (300)	0.15	1.0	23	0.0357
IO	IO	21.4 (300)	0.15	3.0d	23	0.0611

Notes.

The original biofilm was cultured in triplicates in these conditions at pH 8.0. The carriers were transferred 5 times in fresh medium around each week before measuring the denitrifying activities.

^aFrom Payette et al. (2019).

^bReference biofilm cultures.

^cThe C/N ratio was 1.5 in all biofilm cultures.

^dThe exact amount of NaCl added in the IO medium is not known. See Payette et al. (2019) for the IO composition. For comparison, the amount of Na⁺ and Cl⁻ in the ASW medium is 3.2%.

In gray are changed parameters from the reference biofilm cultures.

IO

Instant Ocean medium