Table 5. Changes in the relative transcript levels of genes involved in the nitrogen metabolism in strain GP59 and strain NL23.
| Genes | Ratio TPM Biofilm cultures/TPM pure cultures | ||||
|---|---|---|---|---|---|
| GP59 | NL23 | ||||
| Ref300N-23C | 900N-30C | 0%NaCl | 0%NaCl | Genes | |
| Denitrification | |||||
| narXL | ns | ns | ns | −9.4 | napGH |
| narK1K2GHJI-1 | ns | −7.4 | 2.1 | 8.2 | napEFDABC |
| narK12 | ns | ns | 2.0 | ||
| narGHJI-2 | 2.7 | 2.8 | 3.0 | ||
| nirK | 11 | 4.7 | ns | 49 | nirKV |
| norCBDQ-1 | ns | ns | −2.7 | 5.9 | norCBQDE |
| norRE | ns | ns | −3.1 | ||
| norCBQD-2 | 2.4 | 3.8 | 2.8 | ||
| nosRZDFYL | ns | ns | ns | 5.2 | nosRZDFYLX |
| nnrS (3) | ns | ns | ns | ns | nnrS (2) |
| nsrR (2) | 2.1 | 2.5 | ns | ns | nsrR |
| DnrN | 2.3 | 2.8 | −3.0 | ns | nnrU |
| NOdiox (2) | ns | ns | ns | 4.0 | nnrR |
| Nitrogen assimilatory pathway | |||||
| ns | nasTS | ||||
| NO3/NO2 trp | 6.0 | 7.2 | 17.9 | 7.8 | ntrABC |
| nasAnirBD | 12 | 24 | 42 | 3.3 | nirBAnasA |
| ntrYX | ns | ns | ns | ns | ntrYX |
| glnA | 2.0 | 3.1 | 5.1 | 19 | glnA |
| −2.1 | glnA (3) | ||||
| gltBD: GOGAT | ns | ns | ns | ns | gltBD |
| glnB | ns | ns | ns | 14 | glnB |
| GDH | ns | ns | ns | ns | GDH |
| glnD | ns | ns | ns | −2.9 | glnD |
| glnE | ns | ns | ns | −3.2 | glnE |
| glnK | 18 | 24 | 25 | 22 | glnK |
| NH4 trp | 11 | 11 | 11 | 20 | NH4-trp |
| glnLG | ns | 2.1 | 5.9 | ns | glnLG |
Notes.
Data values are the ratios of the biofilm-culture TPM divided by the planktonic pure-culture TPM. When the ratios were <1, the negative inverse values (−1/ratio) were calculated. Positive ratios mean higher relative transcript levels in the biofilm cultures, and negative ratios higher relative transcript levels in the planktonic pure cultures.
- ns
- no significant changes
- glnA
- glutamine synthetase
- gltBD
- glutamate synthase
- GDH
- glutamate dehydrogenase
- glnB
- nitrogen regulatory protein P-II 1
- glnK
- nitrogen regulatory protein P-II 2
- glnD
- uridylyltransferase
- glnLG
- nitrogen regulation sensor histidine kinase and response regulator
- trp
- transporter
- Diox
- dioxygenase
- nnrS
- involved in response to NO
- nsrR
- NO-sensitive transcriptional repressor
- DnrN
- NO-dependent regulator
- nasAnirBD
- assmilatory nitrate and nitrite reductase
- ntrXY
- Nitrogen regulation proteins
- narXL
- nitrate/nitrite sensor histidine kinase and response regulator
- narK
- nitrate/nitrite transporter
- nosRE
- NO-reductase transcription regulator and activation protein