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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Pestic Biochem Physiol. 2018 Jul 10;150:48–58. doi: 10.1016/j.pestbp.2018.07.003

Figure5. A single cell-based high-content analysis (HCA) of concentration-dependent effects of diazinon on the neutral lipid droplets accumulation (A-D) and FABP4 expression (E-H).

Figure5.

Figure5.

The 3T3-L1 were inoculated in a 96-well plate and treated with diazinon at the doses as indicated. On maturation stage of 8 days, the cells were stained with LipidTOX for neutral lipid (green) and Hoechst 33327 for nuclei (blue). The images were acquired with a 20 × objective and representative image from 49 fields/well were shown. A: vehicle control, B: 1 μM, C: 10 μM, D: 25 μM. Cells were also probed with a specific primary antibody of FABP4 (Cell Signaling), followed by DyLight 650 conjugated secondary antibodies(red). Nuclei were stained with Hoechst 33327 (blue). E: vehicle control, F: 1 μM, G: 10 μM, H: 25 μM. Scale bar is 50 μm