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. 2019 Aug 9;10:1892. doi: 10.3389/fimmu.2019.01892

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Copyright © 2019 Bogen, Hinz, Grzeschik, Ebenig, Krah, Zielonka and Kolmar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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BLI sensorgrams of CEACAM5 binding to the pH-responsive clones K39, K40, CV1, CV2, and CV3 as well as for the parental non-pH-responsive IGKV3-15*01 immobilized on AHC tips. The association was performed with 100 nM CEACAM5 at pH 7.4 in KB. The dissociation was performed in KB at the respective pH value. The depicted lines are colored according to the respective light chain data.