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. 2019 Aug 9;10:1876. doi: 10.3389/fimmu.2019.01876

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Copyright © 2019 Marrella, Dondero, Aiello, Casu, Olive, Regis, Bottino, Pende, Meazza, Caluori, Castriconi and Scaglione.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NB cell-laden hydrogel tumor model. (A) Picture, optical image of NB-loaded alginate spheres and AFM figures showing NB cells clusters within the hydrogels. (B) Representative images of DAPI-stained NB cells nuclei within alginate spheres at different time points and histological sections stained with H&E after 7 days of culture. (C) Quantitative analysis of the morphological parameters of the NB cell-laden alginate spheres to monitor the temporal change in the hydrogel dimension (mean ± SD) up to 1 week of culture.