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Real-time PCR analysis of NB cells recovered from 3D alginate hydrogel. Expression of target genes is normalized to that of the GAPDH reference gene. Data in triplicate are shown. Expression relative fold changes are referred to the gene expression in untreated cells, whose expression has been arbitrarily assigned the value 1. Student's t-test. ns, not significant; *P < 0.05; **P < 0.01, ***P < 0.001, ****P < 0.0001.
