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. 2019 Aug 9;10:1876. doi: 10.3389/fimmu.2019.01876

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Copyright © 2019 Marrella, Dondero, Aiello, Casu, Olive, Regis, Bottino, Pende, Meazza, Caluori, Castriconi and Scaglione.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Constitutive and IFN-γ-induced immunophenotype of NB cells cultured for 7 days in 2D systems. (A) Surface phenotype of NB cell lines cultured a week either in the absence or in the presence of IFN-γ (immunofluorescence and flow cytometry). The anti-NKp46 mAb is used as a negative control. Four independent experiments with mean, 95% confidence intervals, and significance are shown. *p < 0.05. (B) A representative experiment is shown. White profiles refer to cells incubated with isotype-matched controls. Values inside each histogram indicate the MFI.