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. Author manuscript; available in PMC: 2020 Jul 10.
Published in final edited form as: J Vis Exp. 2019 Jul 10;(149):10.3791/59913. doi: 10.3791/59913

Figure 3: Estimation of RNA and protein yields from RIPiT.

Figure 3:

(A) Western blot of the proteins purified from each major step in the RIPiT procedure. (B) Autoradiograph image of RNA footprints from a FLAG-MAGOH:EIF4AIII RIPiT comparing puromycin treated and untreated cells. Red box indicates the RNA footprint size that will ultimately be converted into sequencing libraries. (C) Profile of RNA eluted from RIPiT as in lane 2 in panel B when visualized using a bioanalyzer.