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. Author manuscript; available in PMC: 2020 Jul 10.
Published in final edited form as: J Vis Exp. 2019 Jul 10;(149):10.3791/59913. doi: 10.3791/59913

Figure 5: Test PCR resolved on 8% non-denaturing PAGE.

Figure 5:

Note the aberrantly large PCR products which appear at 14 cycles (red box) and the parallel depletion of primers (red arrow) indicative of overamplification. For this sample, 11 cycles was chosen for the large-scale PCR (blue box).