Skip to main content
. Author manuscript; available in PMC: 2020 Jul 10.
Published in final edited form as: J Vis Exp. 2019 Jul 10;(149):10.3791/59913. doi: 10.3791/59913
Name of Material/ Equipment Company Catalog Number Comments/Description
Anti-FLAG Affinity Gel Sigma A2220
ATP, [γ−32P]- 3000Ci/mmol 10mCi/ml EasyTide, 250μCi PerkinElmer BLU502A250UC
BD Disposable Syringes with Luer-Lok™ Tips (200) Fisher 14–823–435
Betaine 5M Sigma B0300
biotin-dATP TriLink N-5002
biotin-dCTP Perkin Elmer NEL540001EA
Branson Sonifier, Model SSE-1 Branson
CircLigase I VWR 76081–606
DMEM, High Glucose  ThermoFisher 11995–065
DNA load buffer NEB NEB
Dynabeads Protein A LifeTech 10002D
Flp-In-T-REx 293 Cell Line ThermoFisher R78007
GeneRuler Low Range DNA Ladder ThermoScientific FERSM1203
Hygromycin B ThermoFisher 10687010
Mini-PROTEAN TBE Gel 10 well Bio-Rad 4565013
Mini-PROTEAN TBE-Urea Gel Bio-Rad 4566033
miRCAT-33 adapter 5′-TGGAATTCTCGGGTGCCAAGGddC-3′ Any this protocol is only compatible with the Illumina sequencing platform
Mirus transIT-X2 transfection reagent Mirus MIR 6004
Mth RNA ligase NEB E2610S
PE1.0 5′-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC*T-3′ Any this protocol is only compatible with the Illumina sequencing platform
PE2.0 5′-CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATC*T-3′ Any this protocol is only compatible with the Illumina sequencing platform
Phenol/Chloroform/Isoamyl Alcohol (25:24:1, pH 6.7, 100ml) Fisher BP1752I-100
Purple Gel Loading Dye (6x) NEB NEB #7025
Q5 DNA Polymerase NEB M0491S/L
RNase I, E. coli, 1000 units Eppicenter N6901K
SPIN-X column Corning CLS8160–24EA
Streptavidin beads ThermoFisher  60210
Superscript III (SSIII) ThermoScientific 18080044
SybrGold ThermoFisher S11494
T4 Polynucleotide Kinase-2500U NEB M0201L
T4RNL2 Tr. K227Q NEB M0351S
Tetracycline Sigma 87128
Thermostable 5´ App DNA/RNA Ligase NEB M0319S
TruSeq_SE1 5′-pGGCACTANNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT-SPACER 18-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCCTTGGCACCCGAGAATTCCA-3′ Any this protocol is only compatible with the Illumina sequencing platform
TruSeq_SE10 5′-pGGTGTTCNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT-SPACER 18-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCCTTGGCACCCGAGAATTCCA-3′ Any this protocol is only compatible with the Illumina sequencing platform
TruSeq_SE11 5′-pGGTAAGTNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT-SPACER 18-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCCTTGGCACCCGAGAATTCCA-3′ Any this protocol is only compatible with the Illumina sequencing platform
TruSeq_SE12 5′-pGGAGATGNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT-SPACER 18-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCCTTGGCACCCGAGAATTCCA-3′ Any this protocol is only compatible with the Illumina sequencing platform
TruSeq_SE2 5′-pGGGTAGCNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT-SPACER 18-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCCTTGGCACCCGAGAATTCCA-3′ Any this protocol is only compatible with the Illumina sequencing platform
TruSeq_SE35′-pGGTCGATNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT-SPACER 18-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCCTTGGCACCCGAGAATTCCA-3′ Any this protocol is only compatible with the Illumina sequencing platform
TruSeq_SE4 5′-pGGCCTCGNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT-SPACER 18-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCCTTGGCACCCGAGAATTCCA-3′ Any this protocol is only compatible with the Illumina sequencing platform
TruSeq_SE5 5′-pGGTGACANNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT-SPACER 18-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCCTTGGCACCCGAGAATTCCA-3′ Any this protocol is only compatible with the Illumina sequencing platform
TruSeq_SE6 5′-pGGTAGACNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT-SPACER 18-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCCTTGGCACCCGAGAATTCCA-3′ Any this protocol is only compatible with the Illumina sequencing platform
TruSeq_SE7 5′-pGGGCCCTNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT-SPACER 18-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCCTTGGCACCCGAGAATTCCA-3′ Any this protocol is only compatible with the Illumina sequencing platform
TruSeq_SE8 5′-pGGATCGGNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT-SPACER 18-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCCTTGGCACCCGAGAATTCCA-3′ Any this protocol is only compatible with the Illumina sequencing platform
TruSeq_SE9 5′-pGGACTGANNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT-SPACER 18-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCCTTGGCACCCGAGAATTCCA-3′ Any this protocol is only compatible with the Illumina sequencing platform
Typhoon 5 Bimolecular Imager GE Healthcare Life Science 29187191