Anti-FLAG Affinity Gel |
Sigma |
A2220 |
|
ATP, [γ−32P]- 3000Ci/mmol 10mCi/ml EasyTide, 250μCi |
PerkinElmer |
BLU502A250UC |
|
BD Disposable Syringes with Luer-Lok™ Tips (200) |
Fisher |
14–823–435 |
|
Betaine 5M |
Sigma |
B0300 |
|
biotin-dATP |
TriLink |
N-5002 |
|
biotin-dCTP |
Perkin Elmer |
NEL540001EA |
|
Branson Sonifier, Model SSE-1 |
Branson |
|
|
CircLigase I |
VWR |
76081–606 |
|
DMEM, High Glucose |
ThermoFisher |
11995–065 |
|
DNA load buffer NEB |
NEB |
|
|
Dynabeads Protein A |
LifeTech |
10002D |
|
Flp-In-T-REx 293 Cell Line |
ThermoFisher |
R78007 |
|
GeneRuler Low Range DNA Ladder |
ThermoScientific |
FERSM1203 |
|
Hygromycin B |
ThermoFisher |
10687010 |
|
Mini-PROTEAN TBE Gel 10 well |
Bio-Rad |
4565013 |
|
Mini-PROTEAN TBE-Urea Gel |
Bio-Rad |
4566033 |
|
miRCAT-33 adapter 5′-TGGAATTCTCGGGTGCCAAGGddC-3′ |
Any |
|
this protocol is only compatible with the Illumina sequencing platform |
Mirus transIT-X2 transfection reagent |
Mirus |
MIR 6004 |
|
Mth RNA ligase |
NEB |
E2610S |
|
PE1.0 5′-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC*T-3′ |
Any |
|
this protocol is only compatible with the Illumina sequencing platform |
PE2.0 5′-CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATC*T-3′ |
Any |
|
this protocol is only compatible with the Illumina sequencing platform |
Phenol/Chloroform/Isoamyl Alcohol (25:24:1, pH 6.7, 100ml) |
Fisher |
BP1752I-100 |
|
Purple Gel Loading Dye (6x) |
NEB |
NEB #7025 |
|
Q5 DNA Polymerase |
NEB |
M0491S/L |
|
RNase I, E. coli, 1000 units |
Eppicenter |
N6901K |
|
SPIN-X column |
Corning |
CLS8160–24EA |
|
Streptavidin beads |
ThermoFisher |
60210 |
|
Superscript III (SSIII) |
ThermoScientific |
18080044 |
|
SybrGold |
ThermoFisher |
S11494 |
|
T4 Polynucleotide Kinase-2500U |
NEB |
M0201L |
|
T4RNL2 Tr. K227Q |
NEB |
M0351S |
|
Tetracycline |
Sigma |
87128 |
|
Thermostable 5´ App DNA/RNA Ligase |
NEB |
M0319S |
|
TruSeq_SE1 5′-pGGCACTANNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT-SPACER 18-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCCTTGGCACCCGAGAATTCCA-3′ |
Any |
|
this protocol is only compatible with the Illumina sequencing platform |
TruSeq_SE10 5′-pGGTGTTCNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT-SPACER 18-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCCTTGGCACCCGAGAATTCCA-3′ |
Any |
|
this protocol is only compatible with the Illumina sequencing platform |
TruSeq_SE11 5′-pGGTAAGTNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT-SPACER 18-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCCTTGGCACCCGAGAATTCCA-3′ |
Any |
|
this protocol is only compatible with the Illumina sequencing platform |
TruSeq_SE12 5′-pGGAGATGNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT-SPACER 18-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCCTTGGCACCCGAGAATTCCA-3′ |
Any |
|
this protocol is only compatible with the Illumina sequencing platform |
TruSeq_SE2 5′-pGGGTAGCNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT-SPACER 18-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCCTTGGCACCCGAGAATTCCA-3′ |
Any |
|
this protocol is only compatible with the Illumina sequencing platform |
TruSeq_SE35′-pGGTCGATNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT-SPACER 18-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCCTTGGCACCCGAGAATTCCA-3′ |
Any |
|
this protocol is only compatible with the Illumina sequencing platform |
TruSeq_SE4 5′-pGGCCTCGNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT-SPACER 18-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCCTTGGCACCCGAGAATTCCA-3′ |
Any |
|
this protocol is only compatible with the Illumina sequencing platform |
TruSeq_SE5 5′-pGGTGACANNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT-SPACER 18-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCCTTGGCACCCGAGAATTCCA-3′ |
Any |
|
this protocol is only compatible with the Illumina sequencing platform |
TruSeq_SE6 5′-pGGTAGACNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT-SPACER 18-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCCTTGGCACCCGAGAATTCCA-3′ |
Any |
|
this protocol is only compatible with the Illumina sequencing platform |
TruSeq_SE7 5′-pGGGCCCTNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT-SPACER 18-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCCTTGGCACCCGAGAATTCCA-3′ |
Any |
|
this protocol is only compatible with the Illumina sequencing platform |
TruSeq_SE8 5′-pGGATCGGNNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT-SPACER 18-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCCTTGGCACCCGAGAATTCCA-3′ |
Any |
|
this protocol is only compatible with the Illumina sequencing platform |
TruSeq_SE9 5′-pGGACTGANNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT-SPACER 18-CTCGGCATTCCTGCTGAACCGCTCTTCCGATCTCCTTGGCACCCGAGAATTCCA-3′ |
Any |
|
this protocol is only compatible with the Illumina sequencing platform |
Typhoon 5 Bimolecular Imager |
GE Healthcare Life Science |
29187191 |
|