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. Author manuscript; available in PMC: 2019 Aug 16.
Published in final edited form as: Breast Cancer Res Treat. 2009 May 23;120(3):593–601. doi: 10.1007/s10549-009-0422-1

Fig. 3.

Fig. 3

Down-regulation of BRCA1 gene expression by promoter methylation in UACC3199 and HCC38 cells. a Quantitative RT-PCR analysis of BRCA1 mRNA expression in untreated MCF-7, UACC3199 and HCC38 cells. Real-time PCR was performed in triplicate in two independent experiments. Bar shows the mean ± SE. b Western blot analysis of BRCA1 protein expression in untreated and demethylated UACC3199, and HCC38 cells. MCF-7 was used as a positive control and Actin served as a loading control. c BRCA1 mRNA expression in UACC3199 and HCC38 cells after treatment with indicated dose of 5-aza-CdR. d BRCA1 mRNA expression in UACC3199 and HCC38 cells after treatment with indicated dose of zebularine