| Antibodies |
|
|
| vGlut1 (1:1000) |
Synaptic Systems |
Cat# 135 303 |
| NeuN (1:2000) |
Synaptic Systems |
Cat# 266 004 |
| IBA1 (1:500) |
Wako Chemicals |
Cat# 019–19741 |
| C1q (1:500) |
Abcam |
Cat# ab182451 |
| CD40 (1:200) |
Thermo Fisher Scientific |
Cat# MA5–17852 |
| γH2Ax (1:500) |
Millipore |
Cat# 05–636 |
| GAD65 (1:500) |
Abcam |
Cat# ab26113, GAD-6 |
| GAPDH (1:2000) |
Santa Cruz Biotechnology |
Cat# sc-32233, 6C5 |
| c-Fos (1:500) |
Santa Cruz Biotechnology |
Cat# sc-52 |
| ARMET (1:1000) |
Abcam |
Cat# ab67271 |
| Total Tau (1: 1000) |
Santa Cruz Biotechnology |
Cat# sc-5587, H-150 |
| p35/p25 (1:1000) |
In house made |
N/A |
| EGFP (1:1000) |
Thermo Fisher Scientific |
Cat# A-11122 |
| β-Actin (1:3000) |
Abcam |
Cat# ab9485 |
| DNM-1 (1:1000) |
Thermo Fisher Scientific |
Cat# MA5–15285, 3G4B6 |
| Ser774-DNM-1 (1:1000) |
Thermo Fisher Scientific |
Cat# PA5–38112 |
| DNM-3 (1:1000) |
Thermo Fisher Scientific |
Cat# PA1–662 |
| Donkey anti-Rabbit, Alexa Fluor 488 |
Invitrogen |
Cat# A21206 |
| Donkey anti-Mouse, Alexa Fluor 488 |
Invitrogen |
Cat# A21202 |
| Donkey anti-Rabbit, Alexa Fluor 555 |
Invitrogen |
Cat# A-31572 |
| Donkey anti-Rabbit, Alexa Fluor 594 |
Invitrogen |
Cat# A11037 |
| Donkey anti-Goat, Alexa Fluor 594 |
Invitrogen |
Cat# A11058 |
| Donkey anti-Mouse, Alexa Fluor 594 |
Invitrogen |
Cat# A11032 |
| Donkey anti-Guinea pig, Alexa Fluor 647 |
Invitrogen |
Cat# A21450 |
| Donkey anti-Goat, Alexa Fluor 647 |
Invitrogen |
Cat# A21447 |
| Donkey anti-Mouse, Alexa Fluor 647 |
Invitrogen |
Cat# A31571 |
| Donkey anti-Rabbit, Alexa Fluor 647 |
Invitrogen |
Cat# A31573
|
| Experimental Models: Organisms/Strains |
|
|
| Mouse: wild type C57BL/6J |
Jackson Laboratory |
JAX: 000664 |
| B6;CBA-Tg(Camk2a-tTA)1Mmay/J |
Jackson Laboratory |
Stock No: 003010 |
| C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J |
Jackson Laboratory |
Stock No: 005706 |
| 5XFAD: B6SJL-Tg(APPSwFlLon,PSEN1*M146L*L28 6V)6799Vas/Mmjax |
Jackson Laboratory |
Stock No: 34840-JAX |
| Tau P301S-tg: B6;C3-Tg(Prnp-MAPT*P301S)PS19Vle/J |
Jackson Laboratory |
Stock No: 008169 |
| B6.129(Cg)-Fostm1.1(cre/ERT2)Luo/j |
Jackson Laboratory |
Stock No: 021882 |
| Software and Algorithms |
|
|
| MATLAB |
Mathworks |
mathworks.com |
| IMARIS |
Bitplane |
bitplane.com |
| R studio |
|
www.rstudio.com |
| ImageJ |
NIH |
imagej.nih.gov/ij |
| EthoVision XT |
Noldus |
noldus.com |
| TSEsystem |
TSE Systems |
tse-systems.com |
| GraphPad Prism |
GraphPad |
graphpad.com |
| Proteome Discoverer version 1.4.1.14 |
Thermofisher |
tools.thermofisher.com |
| Ingenuity Pathway Analysis |
Qiagen |
qiagenbioinformatics.com |
| SPSS (version 24) |
IBM Analytics |
ibm.com |
| ZEN |
Zeiss |
zeiss.com |
