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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Cancer Discov. 2019 Apr 3;9(6):778–795. doi: 10.1158/2159-8290.CD-18-1138

Fig.1. Activation of TET enzymes in response to extracellular cytokines leads to gain of 5-hmC in the genome.

Fig.1

(A) Lineage uncommitted CD34+ hematopoietic stem/early progenitors were exposed to pleotropic cytokine FLT3L for various times and nuclear lysates were used for determining TET enzyme activity. Data are represented as mean +SEM from three biological replicates. (B, C) Primary erythroid progenitors (basophilic stage) derived after differentiation of CD34+ stem/early progenitors were exposed to stem cell factor (SCF) or erythropoietin (EPO) for various times and nuclear lysates were used for determining TET enzyme activity. Data are represented as mean +SEM from three biological replicates. (D, E, F) Lineage uncommitted CD34+ hematopoietic stem/early progenitors or primary erythroid progenitors were exposed to FLT3L, SCF or EPO as indicated for 30 minutes prior to isolating genomic DNA and determining the 5-hmC content. Data are represented as mean +SEM from three biological replicates.