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. Author manuscript; available in PMC: 2020 Jun 27.
Published in final edited form as: Cell. 2019 May 30;178(1):160–175.e27. doi: 10.1016/j.cell.2019.05.012

Figure 6. Tumor glands are independent “units” in the architecture of primary PDAC tumors.

Figure 6

(A) Representative images of dual-color tissue RNA-ISH of primary human PDACs stained for PRO marker MKI67 (Ki67) and EMT marker FN1. Representative image analysis of tumor glands using quantitative digital pathology software to score single cancer cells in distinct cell phenotypes: DP (Ki67+/FN1+), EMT (−/FN1+), PRO (Ki67+/−) and DN (−/−). Image Bar = 20 μm, Inset Bar = 3 μm. (B-C) Kaplan-Meier survival curves for high vs low DP (Ki67+/FN1+), EMT (Ki67−/FN1+), and PRO (Ki67+/FN1−) cells. (B) Single cell scoring by the total number of cancer cells per tumor (left column) compared to (C) single cell scoring normalized per gland basis (right column). A uniform cutoff of 15% was applied to divide low- vs high-risk patients in each Kaplan-Meier curve.