Figure 3:

Fragments derived from a time course of shrimp and pig Tpm simulated gastric digestion was quantified by LC-MS/MS. Approximately 200 fragments were identified across the digestion time from 5 min to 4hrs. (a) Shrimp and (b) pig Tpm fragments were quantified based on relative abundance across the time series, where abundance is represented by a rainbow palette from blue-low to red-high. Peptides were grouped by k-means clustering. For each cluster, the total number of peptide, and number of peptides overlapping the 5 epitope regions and C terminal half of the protein defined as residues 123–284 are tabulated on the right. Sequence position and relative are shown for (c) shrimp and (d) pig Tpm. Maximum relative abundance of a specific fragment during gastric and intestinal digestion time-course are indicated in red. Grey bands demarcate epitopes.