Figure 4:
Thermal denaturation of Tpm was observed by CD spectroscopy following helical transitions at 222nm. Thermal denaturation (solid lines) and first-derivative plots were calculated (dashed lines) of pig (red), shrimp (black), and rat (blue) Tpm at (a) acidic and (b) neutral conditions. Temperature ranged from 4°C to 70°C or 95°C for neutral and acidic conditions respectively.