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. Author manuscript; available in PMC: 2019 Aug 16.
Published in final edited form as: Mol Microbiol. 2016 Mar 10;100(2):303–314. doi: 10.1111/mmi.13319

Figure 7. Ubiquitin binds to Kog1 in response to temperature upshift.

Figure 7.

A. Wild type yeast cells expressing KOG1-HA (Y985) and ubiquitin were subjected to heat stress for 1 hr. Cell extracts were precipitated with anti-HA antibody and the levels of Kog1-HA and ubiquitin in the cell extracts and precipitates were analyzed by western blotting. B. Wild type yeast cells expressing KOG1-HA (Y985), together with myc-tagged ubiquitin or ubiquitin G76A mutant were subjected to heat stress for 1 hr. Cell extracts were precipitated with anti-HA antibody and the levels of Kog1-HA and myc tagged ubiquitin in the extracts and precipitates were examined by western blotting.

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