(A) Representative plots of flow cytometry analysis of the colonic
lamina propria of indicated mouse strains.
(B) Quantification of flow cytometry analysis according to gating
strategy indicated in A.
(C) Representative plots of flow cytometry analysis of the colonic
lamina propria of
Cx3cr1cre:Il10rafl/fl mice
(left). Quantification of flow cytometry analysis (right).
(D) Schematic of T cell depletion protocol
(E) Quantification of flow cytometry analysis of mesenteric LN
indicating efficient ablation of CD4+ T cells, but not
CD8+ T cells.
(F) Representative plots of flow cytometry analysis of the colonic
lamina propria of [
Cx3cr1cre:Il10rafl/fl > WT
] BM chimeras.
(G) Representative immunofluorescence images of colon sections of [
Il10rafl/fl > WT ] BM chimeras, [
Cx3cr1cre:Il10rafl/fl > WT
] BM chimeras and [ Cx3cr1cre:Il10rafl/fl
> WT ] BM chimeras treated with the anti-CD4 regimen.
(H) qRT-PCR analysis of il22,
cxcl1 and cxcl5 expression in whole tissue
extracts of colons of indicated BM chimeric mice.
Data are collected from two independent experiments, n>=3 in each