(A) Schematic of neutrophil depletion protocol
(B) Representative plots of flow cytometry analysis of the colonic
lamina propria of [
Cx3cr1cre:Il10rafl/fl > WT
] BM chimeras treated with IgG control or anti Ly6G antibody (left).
Quantification of flow cytometry analysis (right).
(C) Representative immunofluorescence images of the colonic tissue
of [ Cx3cr1cre:Il10rafl/fl > WT ]
BM chimeras treated with IgG control or anti Ly6G antibody.
(D) qRT-PCR analysis of Nos2 expression in whole
tissue extracts of colons of indicated BM chimeric mice.
(E-F) Volcano plot of statistical significance (log10 p-value)
against log 2 ratio of macrophages isolated from colonic tissue of
[Cx3cr1cre:Il10rafl/fl >
WT ] BM chimeras treated with IgG control
(F) or anti Ly6G antibody (E) and [Il10rafl/fl
> WT ] BM chimeras, based on RNA-seq data. Significantly up or down
regulated genes (fold change>2, adj-p value<0.05) are in black,
relevant pro inflammatory up-regulated genes are highlighted in red.
Data in A-D are collected from two independent experiments, n>=3 in
each