Figure 7. Neutrophil depletion ameliorates colitis in Cx3cr1^cre:Il10ra^fl/fl BM chimeras.

(A) Schematic of neutrophil depletion protocol

(B) Representative plots of flow cytometry analysis of the colonic lamina propria of [ Cx3cr1^cre:Il10ra^fl/fl > WT ] BM chimeras treated with IgG control or anti Ly6G antibody (left). Quantification of flow cytometry analysis (right).

(C) Representative immunofluorescence images of the colonic tissue of [ Cx3cr1^cre:Il10ra^fl/fl > WT ] BM chimeras treated with IgG control or anti Ly6G antibody.

(D) qRT-PCR analysis of Nos2 expression in whole tissue extracts of colons of indicated BM chimeric mice.

(E-F) Volcano plot of statistical significance (log10 p-value) against log 2 ratio of macrophages isolated from colonic tissue of [Cx3cr1^cre:Il10ra^fl/fl > WT ] BM chimeras treated with IgG control

(F) or anti Ly6G antibody (E) and [Il10ra^fl/fl > WT ] BM chimeras, based on RNA-seq data. Significantly up or down regulated genes (fold change>2, adj-p value<0.05) are in black, relevant pro inflammatory up-regulated genes are highlighted in red.

Data in A-D are collected from two independent experiments, n>=3 in each