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. Author manuscript; available in PMC: 2020 Aug 9.
Published in final edited form as: J Mol Biol. 2019 Jun 4;431(17):3261–3274. doi: 10.1016/j.jmb.2019.05.044

Figure 2.

Figure 2.

Liposome disruption and fluorophore release by PCV2 VLP and components of the capsid protein. The fraction of fluorophore release as compared to the liposome disruption by addition of 1% triton x-100 is represented. Red and blue shades represent fluorescence traces of fluorophore release from 200 nm DOPC:DOPG (75:25) liposomes at pH 7 and 5 respectively. a) VLP, b) capsid protein (CPfl), c) ARM peptide, d) VLP ARM, e) C-terminus peptide. Black curve in panel b and c represent fluorescence traces of fluorophore release from DOPC:DOPG:DOPG-PEG2000 (75:20:5) by capsid protein (CPfl) and ARM peptide, respectively. f) SDS-PAGE of the liposome pelleting assay to assess the association of ARM peptide, Lane 1 and 2: VLP supernatant at pH 7 and 5, Lane 3 and 4: CPfl supernatant at pH 7 and 5, Lane 5 and 6: ARM peptide supernatant at pH 7 and 5, Lane 7 and 8: VLP with liposome at pH 7 and 5, Lane 9 and 10: CPfl with liposome at pH 7 and 5, Lane 11 and 12: ARM peptide with liposome at pH 7 and 5.