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. 2019 Aug 16;14(8):e0221156. doi: 10.1371/journal.pone.0221156

Fig 2. RT-PCR analysis of voltage gated sodium channel isoforms in ND7/23 cells.

Fig 2

(A) Expression of NaV1.7, NaV1.8, NaV1.9 sodium channel α-subunits in ND7/23 transcriptome. DNA marker run on non-adjacent lane on same gel (S2 Fig). Expected amplicon sizes were respectively (in bp), 656, 521, and 564. The + and–indicate presence and absence of cDNA in PCR reaction. Gapdh, Glyceraldehyde 3-phosphate dehydrogenase; M, DNA size marker (100 bp). (B), Expression of NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.5, NaV1.6 sodium channel α-subunits. Expected amplicon sizes were respectively (in bp), 683, 674, 681, 368, 589, and 662. (C) Expression of sodium channel auxiliary subunits β1, β2, β3, β4, in ND7/23 transcriptome. Expected amplicon sizes were respectively (in bp), 657, 648, 648, and 687. Gels are representative of at least three independent experiments.