Skip to main content
. 2019 Aug 16;14(8):e0221156. doi: 10.1371/journal.pone.0221156

Fig 4. Genomic origin of sodium channels in ND7/23 cells.

Fig 4

(A) RT-PCR of NaV1.8 from ND7/23 cDNA synthesized with oligo-dT or NaV1.8-specific (SP) reverse primer. DNA marker run on non-adjacent lane on same gel (S2 Fig). Expected amplicon size in mouse or rat is 521 bp. The–symbol indicate absence of cDNA in PCR reaction. ND7, ND7/23; rDRG, rat dorsal root ganglion; M, DNA size marker (100 bp). (B) EcoRI restriction enzyme cleavage of NaV1.7 amplicons from ND7/23 cells. Mouse NaV1.7 sequence does not contain the EcoRI restriction site that rat Nav1.7 sequence harbors within. The–and + symbols indicate absence or addition of EcoRI enzyme to NaV1.7 amplicons. (C) RT-PCR of ND7/23 cDNA synthesized with oligo-dT or NaV1.9-specific (SP) reverse primers using mouse-Nav1.9-b primer set (606 bp, different primers from that used in Fig 2A) and rat-specific NaV1.9 primer set. Gels are representative of at least three independent experiments.