Table 1. Nucleotide sequences and PCR conditions for the detection of rodent-borne bacteria in the rodent tissue.

Bacteria	Target gene	Primer name	Nucleotide sequence (5′-3′)	Product size (bp)	PCR profile (°C/s)				Reference
					Denaturation	Annealing	Extension	Cycles
Orientia tsutsugamushi	rrs	ot-16sRF1	AGGGATGATAATGACAGTACCTACAG	199	94/60	57/30	72/45	36	In this study
		ot-16sRR1	CCTCTACCATACTCTAGCCTAACAG
	56 kDa	56BO-144F	YGYAGAATCTRCTCGCTTGG	1250	94/60	60/60	72/60	35	In this study
		56BO-1395R	AGCTAMCCCTRCACCAABAC
		56BO-406F	CCWCCTCARCCTACTATRATGC	680	94/30	61/30	72/45	30
		56BO-1088R	GCWGCTGCTRCTGCTTCTTG
Anaplasma phagocytophilum	groEL	GRO607F	GAAGATGCWGTWGGWTGTACKGC	688	95/30	54/30	72/60	30	[12]
		GRO1294R	AGMGCTTCWCCTTCWACRTCYTC
		GRO677F	ATTACTCAGAGTGCTTCTCARTG	445	95/30	57/30	72/60	30
		GRO1121R	TGCATACCRTCAGTYTTTTCAAC
	ankA	ANK-F1	GAAGAAATTACAACTCCTGAAG	705	95/30	53/30	72/60	35	[13]
		ANK-R1	CAGCCAGATGCAGTAACGTG
		ANK-F2	TTGACCGCTGAAGCACTAAC	664	95/30	52/30	72/60	5
		ANK-R2	ACCATTTGCTTCTTGAGGAG		95/30	54/30	72/60	25
Leptospira interrogans	rpoB	rpoB-1889F	GTTCCAACATGCAACGYCAR	1649	94/60	52/60	72/60	35	In this study
		rpoB-3537R	GTTGAAGGATTCRGGRATAC
		rpoB-2438F	TYATGCCKTGGGAAGGWTAC	1023	94/30	56/30	72/45	30
		rpoB-3460R	GCATRTCRTCKGACTTGATG
	LipL32	hap1-435F	GGGAATACGTAGAMGTTCG	1435	94/60	56/60	72/60	35	In this study
		hap1-1870R	GTTTATAGTAGGTTGAAGCTTG
		L-hap1-217F	CCGTGATTTTCCTAACTAAGG	848	94/30	58/20	72/45	30	[14]
		L-hap1-218R	CAGATTACTTAGTCGCGTCAGA
	gyrB	LeptoB2F	TGAGCCAAGAAGAAACAAGCTACA	500	94/30	59/30	72/45	35	[15]
		LeptoB504R	MATGGTTCCRCTTTCCGAAGA