Fig 2. Schematic representation of generation of transgenic Drosophila.

Novel Newt candidate genes were used to create 5 transgenic flies. Each fly had a single copy of newt candidate gene (C1, C2, C3, C4 or C5) inserted at chromosome III. After generating transgenic flies, genotyping was performed to check if Newt genes have been properly incorporated in Drosophila genome.