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(A) Western blot performed using total protein lysates from EZH2-mutated (SU-DHL-10, WSU-DLCL-2, KARPAS-422, Carnaval) and wild-type EZH2 (HT, U-2932 R2, U-2932 R1, DG-75, CA-46, BLUE-1, BL-2 and BL-41) cell lines showing an elevated H3K27me3 in EZH2-mutated cell lines as compared to wild-type EZH2 cell lines. (B) Densitometric quantification of EZH2 and H3K27me3 normalized for GAPDH.
