Fig 2. Quantitative estimation of Leishmania infantum in blood samples from infected dogs.
kDNA qPCR assay showing the quantification of all positive samples from A; August 2017, B; CCZ (April 2018) and C; in the field (April 2018). All positive samples previously determined by conventional PCR were qualitatively analysed by qPCR to determine parasite loads of each positive dog. Range of parasite load; 0.4 parasites mL1 to 853 parasites mL-1. White bars; asymptomatic dogs, grey bars; oligosymptomatic dogs and black bars; symptomatic dogs. Parasite load is per mL of blood. For clarity parasite load is given for all dogs in 2A and 2B but is excluded from dogs with similar loads in 2C. Dog identification number is given on the X-axis.