Fig 10. Total α-tubulin and β-tubulin protein levels in Tuba1a mutants.

Immunoblotting for pan-α (A-D) and pan-β (E-H) tubulin in Tuba1a^d4304 (A,B,E,F) and Tuba1a ^quas (C,D,G,H) wild-type, heterozygous and mutant embryos. Protein levels were quantified by comparison to GAPDH loading control (A,C,E,G) and REVERT total protein stain (B,D,F,H). (A) Colors indicate results from five different technical replicates. (B-H) Colors indicate littermates (technical replicates are shown for A-C,E,G). For Tuba1a^d4304, n = 3 animals of each genotype, for Tuba1a^quas, wild-type n = 3, heterozygous n = 2, and mutant n = 5. (t = presumed tubulin protein, a = presumed actin protein).