Skip to main content
. 2019 Jul 2;117(3):445–452. doi: 10.1016/j.bpj.2019.06.022

Figure 2.

Figure 2

Photoinhibition of membrane fusion by influenza virus labeled with R18 or TR-DHPE dyes. Lipid-mixing efficiency is plotted for virus labeled with different concentrations of (a) R18 or (b) Texas Red dye and exposed to the indicated illumination intensities for 6 min before measurement. Photoinhibition is apparent at high dye concentrations and more marked for R18 than Texas Red. Efficiency is calculated as number of virions undergoing lipid mixing/total number of bound virions observed. Error bars are plotted as SDs across fields of view for unilluminated samples and bootstrapped SDs across individual viruses for illuminated samples. Illumination intensities are reported as fraction of maximal LED intensity. Measured intensities are given in the Materials and Methods. To see this figure in color, go online.