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. 2019 Jul 2;117(3):553–562. doi: 10.1016/j.bpj.2019.06.025

Figure 5.

Figure 5

IRSp53 I-BAR domain induced flower-like GUV membranes. (A and B) Shown are the representative confocal images of a GUV in the presence of IRSp53 I-BAR domain. Confocal images were taken at the equator of the GUV (A) and at the top of the GUV (B). Arrows in (A) indicate some membrane indentations. (C) Maximal intensity projection of the same GUV is shown in (A) and (B). (D) Maximal intensity projection of a flower-like GUV in the presence of IRSp53 I-BAR domain is shown. Protein concentrations are as follows: (A–C) 0.02 μM (70% unlabeled and 30% AX488-labeled I-BAR domain) and (D) 0.1 μM AX488-labeled I-BAR domain. Scale bars, 5 μm. To see this figure in color, go online.