TABLE I. Primers used in quantitative real-time polymerase chain reaction (PCR).
| Target gene (ID) | Sequence 5’- 3’ (Forward and reverse) | Tm | GC % | Amplicon length (bp) |
| GAPDH (LbrM.30.2950) | CGACCAGGACCTTATTGGTAAA | 63.7 | 45.5 | 100 |
| CACCGTATCGTGCTTCATCT | 62.7 | 50 | ||
| MRPA (LbrM.23.0280) | CTTCTCCATGTGCTCCACTATC | 62.6 | 50 | 103 |
| CACCTGCATCAGCTTGTAGTA | 60.5 | 47.6 | ||
| AQP1 (LbrM.31.0020) | GGTATCACGACAGGTATCAACTC | 61.3 | 47.8 | 84 |
| CCAGAGCATGGCTGTGAATA | 63.8 | 50 | ||
| GSH1 (LbrM.18.1700) | GGGTGGCTTCTATGGACTTATAC | 61.5 | 47.8 | 100 |
| CATGGACAGGAACCTCAAGTAG | 62.5 | 50 | ||
| ABCG2 (LbrM.06.0080) | CTGAGTTTCCCGTGCAGATT | 64.2 | 50 | 107 |
| CACACCGCAGTAGTAGAAGAAC | 60.4 | 50 | ||
| ABCI4 (LbrM.33.3540) | CTGTAGACGAAGCGGGTATTT | 62 | 47.6 | 135 |
| CTAGGCGATGAGACACCATAAC | 62.4 | 50 | ||
| AMR56 (LbrM.20.0200) | GAGAACGGACAACCCAGTTT | 62.9 | 50 | 105 |
| CCACTTCTTCTGCTGTGAGTT | 61.1 | 47.6 | ||
| ARM58 (LbrM.20.0210) | CCCAAGGGCTTTCACCTAAA | 64.9 | 50 | 103 |
| AGCGGTAGATCTTGTCGTATTG | 62 | 45.5 |
GAPDH: glycosomal glyceraldehyde 3-phosphate dehydrogenase; MRPA: multidrug resistant protein A (ABC transporter ABCC3, also known as PGPA); AQP1: aquaglyceroporin 1; GSH1: gamma-glutamylcysteine synthetase; ABCG2: ATP-binding cassette protein subfamily G, member 2; ABCI4: ATP-binding cassette protein subfamily I member 4; ARM56 and ARM58: antimony resistance marker of 56 and 58 kDa. bp: base pair. Tm (melting temperature) was calculated at default settings of 0.25 mM oligo concentration and 50 mM Na. Primers were designed using PrimerQuest (www.idtdna.com/site/account/login?returnurl=%2FPrimerquest%2FHome%2FIndex), rechecked with OligoAnalyzer (https://www.idtdna.com/calc/analyzer) and OligoEvaluator (Guimarães et al.,(1,2) Rugani et al.(3)).