TABLE II. Single nucleotide polymorphisms (SNPs) of the AQP1 gene of Leishmania braziliensis field isolates and M2903 reference strain.
| Strain | Lesion | DNA | Aminoacid |
| M2903 | Typical | A372G | S |
| T394C | S | ||
| G447A | S | ||
| T449C | V150A | ||
| 346 | Typical | A372G | S |
| T394C | S | ||
| G447A | S | ||
| T449C | V150A | ||
| 426 | Typical | A372G | S |
| T394C | S | ||
| G447A | S | ||
| T449C | V150A | ||
| 330 | Atypical | A372G | S |
| T394C | S | ||
| T449C | S | ||
| A516C | S | ||
| 340 | Atypical | A372G | S |
| T394C | S | ||
| T449C | S | ||
| A516C | S |
Red: SNP exclusive from AT lesion-derived L. braziliensis; blue: SNP present only in typical lesion-derived parasites and shared with M2903, but differs from reference genome of M2904; green: SNPs present in AQP1 from field isolates, shared with M2903, but absent in M2904. S: silent mutation. Primers AQP1F and AQP1R were used for amplification of fragment, and SP6 and T7 for sequencing.