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. 2019 Aug 16;8:e46321. doi: 10.7554/eLife.46321

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© 2019, LeMessurier et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 1. — (A) Experimental time line and example enrichment objects. (B) Schematic of whisker stimulus train and barrel map showing the 3 × 3 array of stimulated whiskers in gray. (C) Example ΔF/F traces from 3 ROIs imaged simultaneously in L2/3 of one column from a Drd3-Cre mouse. Gray bars are deflections of each indicated whisker. (D) Whisker receptive fields for the 3 cells in (C). Top, ΔF/F traces (shown as grayscale after baseline subtraction) for each trial for the nine whiskers (C1 to E3), aligned to stimulus onset (St). Bottom left, Mean ΔF/F trace for each whisker. Vertical bars are whisker deflections. The two strongest whiskers are shown in black and blue, respectively. Bottom right, Median ΔF/F averaged over 1 s response window. (E) Localization of one L2/3 imaging field (box) in a Drd3-Cre mouse relative to column boundaries in a cytochrome oxidase-stained section through L4 (top). Bottom, projection of barrel boundaries onto this imaging field. (F) Comparison of receptive fields measured by simultaneous GCaMP6s imaging and loose-seal cell-attached spike recording. Top: One example L2/3 neuron with its spiking receptive field shown as a peristimulus time histogram for each whisker (center), and its imaging receptive field from ΔF/F (right). Bottom: Average imaging and spiking receptive fields for each whisker-responsive neuron (n = 10). Shading is SEM.

Figure 1—figure supplement 1. — (A) Experimental time line and example enrichment objects. (B) Schematic of whisker stimulus train and barrel map showing the 3 × 3 array of stimulated whiskers in gray. (C) Example ΔF/F traces from 3 ROIs imaged simultaneously in L2/3 of one column from a Drd3-Cre mouse. Gray bars are deflections of each indicated whisker. (D) Whisker receptive fields for the 3 cells in (C). Top, ΔF/F traces (shown as grayscale after baseline subtraction) for each trial for the nine whiskers (C1 to E3), aligned to stimulus onset (St). Bottom left, Mean ΔF/F trace for each whisker. Vertical bars are whisker deflections. The two strongest whiskers are shown in black and blue, respectively. Bottom right, Median ΔF/F averaged over 1 s response window. (E) Localization of one L2/3 imaging field (box) in a Drd3-Cre mouse relative to column boundaries in a cytochrome oxidase-stained section through L4 (top). Bottom, projection of barrel boundaries onto this imaging field. (F) Comparison of receptive fields measured by simultaneous GCaMP6s imaging and loose-seal cell-attached spike recording. Top: One example L2/3 neuron with its spiking receptive field shown as a peristimulus time histogram for each whisker (center), and its imaging receptive field from ΔF/F (right). Bottom: Average imaging and spiking receptive fields for each whisker-responsive neuron (n = 10). Shading is SEM.