Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Caenorhabditis elegans) | LST-1 | UniprotKB: P91820_(CAEEL) | ||
| Gene (Caenorhabditis elegans) | FBF-2 | UniprotKB: Q09312_(CAEEL) | ||
| Strain, strain background (Saccharomyces cerevisiae)) | L40 | ATCC | Cat. #: MYA-3332 | Yeast 2-hybrid strain |
| Strain, strain background (Escherichia coli) | DH5-alpha | Thermo Fisher | Cat. #: 18265017 | Chemically competent cells |
| Strain, strain background (Escherichia coli) | BL21-CodonPlus (DE3)-RIL | Agilent | Cat. #: 230245 | Competent cells |
| Recombinant DNA reagent | pACT2 (plasmid) |
PMID: 21372189 | GenBank Accession #: U29899 | Yeast two-hybrid expression vector with Gal4 activation domain fusion |
| Recombinant DNA reagent | pBTM116 (plasmid) |
Clonetech | Vojtek et al., 1993 | Yeast two hybrid vector with LexA DNA binding ORF |
| Recombinant DNA reagent | pSMT3 (plasmid) |
provided by Dr. Christopher Lima | Mossessova and Lima (2000) | Encodes an N-terminal His6-SUMO fusion tag followed by a TEV protease cleavage site |
| Recombinant DNA reagent | pGEX4T-3 (plasmid) |
GE Healthcare | Cat. #: 27-4583-01 | Bacterial vector for expressing fusion proteins with a thrombin site |
| Recombinant DNA reagent | pMAL-C2T (plasmid) |
New England Biolabs | Accession #: JF795283 | Bacterial vector for cytoplasmic expression of maltose-binding protein fusion |
| Sequence-based reagent | Yeast tRNA | Thermo Fisher | Cat. #: 15401011 |
Carrier for nucleic acid precipitation |
| Peptide, recombinant protein | TURBO DNase | Thermo Fisher | Cat. #: AM2238 |
|
| Peptide, recombinant protein | ImProm-II reverse transcription reaction | Promega | Cat. #: A3803 | |
| Peptide, recombinant protein | GoTaq reaction | Promega | Cat. #: M7123 | |
| Peptide, recombinant protein | T4 polynucleotide kinase | New England Biolabs | Cat. #: M0201S | |
| Peptide, recombinant protein | lysozyme | Thermo Fisher | Cat. #: 89833 |
|
| Commercial assay or kit | β-Glo reagent | Promega | Cat. #: E4720 | |
| Commercial assay or kit | Phusion High- Fidelity PCR Kit | Thermo Fisher | Cat. #: F553S | |
| Commercial assay or kit | AmpliScribe T7-Flash Transcription Kit | Lucigen | Cat. #: ASF3507 | |
| Chemical compound, drug | EDTA-free Protease Inhibitor | Roche | Cat. #: 11836170001 | |
| Chemical compound, drug | Amylose resin | New England Biolabs | Cat. #: E8021S | |
| Chemical compound, drug | Glutathione agarose resin | Gold Biotechnology | Cat. #: G-250 | |
| Chemical compound, drug | Ni-NTA resin | Qiagen | Cat. #: 30210 | |
| Chemical compound, drug | reduced glutathione | Sigma-Aldrich |
Cat. #: G4251 | |
| Chemical compound, drug | Glutathione magnetic beads | Thermo Fisher | Cat. #: 78602 |
|
| Software, algorithm | HKL2000 | http://www.hkl-xray.com/ | Otwinowski and Minor, 1997 | |
| Software, algorithm | Phaser | http://www.ccp4.ac.uk/html/phaser.html | McCoy et al., 2007 | |
| Software, algorithm | Phenix | https://www.phenix-online.org | Adams et al., 2010 | |
| Software, algorithm | Coot | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot | Emsley and Cowtan, 2004 | |
| Software, algorithm | MEME | http://meme-suite.org/ | Bailey et al., 2006 | |
| Software, algorithm | Enrichr |
https://amp.pharm.mssm.edu/Enrichr/
|
Kuleshov et al., 2016 | |
| Software, algorithm | ImageQuant Version 5.1 | GE Healthcare | ||
| Software, algorithm | GraphPad Prism 7 | GraphPad | ||
| Software, algorithm | Matlab R2008a | MathWorks |