Table 2. Frequency of pseudotime expression patterns for melanogaster-specific fixed de novo genes and melanogaster-specific duplicate genes.

For genes in Figure 4C and D, we counted the number of genes showing a strong bias for early pseudotime, late pseudotime, mid-pseudotime, or a bimodal expression pattern. Fixed de novo genes are most frequently biased towards mid-pseudotime and the plurality of melanogaster-specific child duplicate genes show a bimodal expression pattern. Pseudotime expression plots of the parent-child duplicate gene pairs used in this analysis are in Figure 4—figure supplement 1. Proportions are rounded to two decimal places and may not add up to 1.

Pattern	Fixed de novo proportion	Parental duplicate proportion	melanogaster-specific child duplicate proportion
Early	0.26	0.37	0.14
Mid	0.62	0.00	0.21
Late	0.11	0.63	0.21
Bimodal	0.01	0.00	0.43