Table 2. Characteristics of the two presented datasets.
BDA: biotinylated dextran amines
| Dataset | Section number | Anatomical region | Tracer | Injection site | Primary antibody | Secondary antibody | EM size (μm x μm) | EM dwell time (ns) | Pixel size (nm) |
|---|---|---|---|---|---|---|---|---|---|
| 1 | 507 | HVC | Alexa 488FITCTexas red | RAAreaXAvalanche | Rat anti-488Mouse anti-FITCGoat anti-rhodamine | 488 anti-rat647 anti-mouse546 anti-rhodamine | 275 x 205 | 820 | 8 |
| 2 | 203 | Dorsal RA | BDA | Caudal RA | mouse anti-BDA | 647 anti-mouse | 185 x 140 | 6000 | 8 |