Figure 5.

Influence of NaCl concentration (vertical axis) and velocity (horizontal axis) on the overstretching plateau of RNA-DNA hybrids. The percentage of the smooth plateau is marked by a color code as given at the right of the figure. For pulling velocities up to 500 nm/s, the percentage of smooth plateau remains less than 50% whatever the NaCl or MgCl₂ concentration is, whereas for 750 nm/s, the smooth plateau becomes dominant at the highest NaCl concentration used (1 M), both in the absence (circles) or presence (squares) of 10 mM MgCl₂. The data for dsDNA (stars) were taken from (8). To see this figure in color, go online.