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. Author manuscript; available in PMC: 2019 Aug 16.
Published in final edited form as: J Colloid Interface Sci. 2018 Dec 4;538:449–461. doi: 10.1016/j.jcis.2018.12.014

Figure 4.

Figure 4

Inhibitory effects of chronic piezoelectric stimulation on U-87 proliferation by testing different AbBTNP concentrations (100 μg/ml and of 10 μg/ml, the latter corresponding to the concentration of nanoparticles crossing the BBB model after a 72 h treatment). a) CLSM analysis of Ki-67 proliferation marker on control cultures, AbBTNPs-treated cells, US-stimulated cells, and on AbBTNPs+US treated cultures. b) Histogram reporting Ki-67+ nuclei (%). c) Time-lapse Ca2+ imaging in response to plain US and to AbBTNPs+US (10 μg/ml). Images at the top show F/F0 signal of cells after 5 min of US (top left) and AbBTNP+US (top right) stimulations. At the bottom, the graph reports F/F0 traces of cultures stimulated with US (in red) and with US+AbBTNP (in black). * p < 0.05.