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. Author manuscript; available in PMC: 2019 Aug 16.
Published in final edited form as: J Colloid Interface Sci. 2018 Dec 4;538:449–461. doi: 10.1016/j.jcis.2018.12.014

Figure 5.

Figure 5

Nanoparticle-assisted piezoelectric stimulation (AbBTNPs+US) improves anticancer efficacy of temozolomide (TMZ). a) WST-1 assay on U-87 cells incubated for 24 h and 72 h with different concentrations of drug (0-400 μg/ml; data are normalized and expressed as percentage of WST-1 absorbance values measured at 24 h on control cultures). b) WST-1 assay respectively performed on control cultures, cultures incubated with 10 μg/ml AbBTNPs, cultures incubated with 50 μg/ml TMZ, cultures incubated with 50 μg/ml TMZ and 10 μg/ml AbBTNPs, cultures chronically stimulated with US, cultures stimulated with US in the presence of 10 μg/ml AbBTNPs, cultures stimulated with US in the presence of 50 μg/ml TMZ, and, finally, cultures stimulated with US in the presence of 10 μg/ml AbBTNPs and of 50 μg/ml TMZ. c) CLSM imaging of Ki-67 and p53 expression in the different experimental conditions. The histograms reporting the Ki-67+ and p53+ nuclei are respectively showed in d) and e). * p < 0.05.