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. Author manuscript; available in PMC: 2019 Aug 16.
Published in final edited form as: Nitric Oxide. 2018 Apr 17;77:35–43. doi: 10.1016/j.niox.2018.04.005

Fig 3. Inconsistent expression of nNOS in striated muscles of C57/Bl6 mice.

Fig 3

A: 50 μg of protein from detergent solubilsates of eight striated muscles taken from C57BL/6-mice were subjected to immunoblot analysis with a polyclonal anti-nNOS antibody (anti-C-terminus). B: The blot matrices were stained with Ponceau Red after immunoblotting for loading control. C: The nNOS-immunoreactive bands in the muscle solubilsates were densitometrically quantified and normalized to protein loading assessed by Ponceau S-staining in order to depict the nNOS level in each muscle. The concentration of nNOS bands in TA muscle was set as 100%. Mean values ± SD, n=4. D: The expression ratio between the 160-kDa (alpha)- and 140-kDa (beta)-isoforms of nNOS in striated muscles of mice, as determined by densitometry of the nNOS-bands after immunoblotting. Mean values ± SD, n=4.