Fig. 2. Mode of delivery and detection of vaginal bacteria by 16S rRNA amplicon sequencing.
a) Concordant detection of vaginal lactobacilli and a combination of all vaginosis associated bacteria by both Qiagen (x-axis) and Mpbio (y-axis) results in Spearman’s rho correlation coefficients of 0.37 and 0.59, respectively when analysing the upper right quadrant only (>0.1%). b,c) Comparisons with vaginally associated bacteria and mode of delivery. Mann-Whitney U tests were used where values below 1% are regarded as 0%. * P < 0.05, *** P < 0.001. Scatterplots are in Extended Data Fig. 6. Percent read count based on the higher value for given species using Qiagen or Mpbio DNA isolation kit (using all 498 samples).