Table 3.

Comparison of assay sensitivities between 5-plex and duplex reactions on clinical positive samples

		IAV clinical sample		ICV clinical sample		IDV clinical sample
		Duplex	5-plex	Duplex	5-plex	Duplex	5-plex
Correlation coefficient R2		0.998	0.993	0.997	0.990	0.996	0.996
PCR efficiency (E)		90.12%	90.01%	95.22%	94.73%	90.69%	99.58%
Ct on serial dilutions*	10−1	21.03	21.00	22.61	22.62	20.52	20.43
	10−2	24.40	24.24	26.06	26.09	23.53	23.66
	10−3	27.91	27.73	29.61	29.52	26.95	26.95
	10−4	31.66	31.20	33.19	33.19	31.04	30.31
	10−5	35.14	34.55	36.25	36.03	34.34	33.45
	1/2*10−5	36.30	35.90	37.52	37.35	35.54	34.31
	1/4*10−5	37.42	37.17	38.46	38.87	36.24	35.57
	1/8*10−5	38.52	39.07	N/A	N/A	38.1	37.2
Ct difference between assays		0.33		0.13		0.58