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. Author manuscript; available in PMC: 2020 Aug 15.
Published in final edited form as: Cell Chem Biol. 2019 Jun 6;26(8):1143–1158.e6. doi: 10.1016/j.chembiol.2019.05.004

Figure 7. Effects of wogonoside on angiogenesis in vivo.

Figure 7.

Matrigel containing saline injection (control group) or MDA-MB-231 cells and MDA-MB-468 cells was injected subcutaneously to assess angiogenesis in vivo. (A) Macroscopic appearance of matrigel plugs isolated from each group of mice (n=5). (B) The whole-mount of CD31 staining was viewed by laser scanning confocal microscope. (C) The hemoglobin content in matrigel plugs was determined. (D) The expression of CD31 in MDA-MB-231 cells xenograft in nude mice was tested by immunochemistry. (E) Effect of VEGF neutralizing antibody (10 μg/ml) on the tube formation of HUVECs induced by CM from MDA-MB-231 or MDA-MB-468 cells was tested by endothelial cell tube formation assay. (F) Effect of VEGF neutralizing antibody (10 μg/ml) on the enhanced angiogenesis induced by MDA-MB-231 cells or MDA-MB-468 cells (1×106 cells/CAM) was tested by chicken chorioallantoic membrane (CAM) assay. The comparisons were made relative to MDA-MB-231 or MDA-MB-468 cells group and the significance of the difference is indicated as *P value < 0.05 and **P value < 0.01.